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rabbit anti tph2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti tph2
    Rabbit Anti Tph2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+tph2/TPH2+XP+Rabbit+mAb/pmc12217342-55-14-16
    Average 93 stars, based on 17 article reviews
    rabbit anti tph2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies: Chicken anti-MAP2 (PA1-10005, Invitrogen); Rabbit anti-Caspase-3 (#9661, Cell Signaling Technology); Rabbit anti-Iba-1 (#17198, Cell Signaling Technology); Rabbit anti-GFAP (#12389, Cell Signaling Technology); Rabbit anti-TPH2 (#51124, Cell Signaling Technology); Rabbit anti-c-Fos (#2250, Cell Signaling Technology). .. Secondary antibodies: Goat anti-chicken, Alexa Fluor 555 (A-21103, Invitrogen); Goat anti-rabbit, Alexa Fluor 488 (A-21428, Invitrogen).

    Flow Cytometry:

    Article Title: Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies: Chicken anti-MAP2 (PA1-10005, Invitrogen); Rabbit anti-Caspase-3 (#9661, Cell Signaling Technology); Rabbit anti-Iba-1 (#17198, Cell Signaling Technology); Rabbit anti-GFAP (#12389, Cell Signaling Technology); Rabbit anti-TPH2 (#51124, Cell Signaling Technology); Rabbit anti-c-Fos (#2250, Cell Signaling Technology). .. Secondary antibodies: Goat anti-chicken, Alexa Fluor 555 (A-21103, Invitrogen); Goat anti-rabbit, Alexa Fluor 488 (A-21428, Invitrogen).

    Magnetic Resonance Imaging:

    Article Title: Nanobubble-actuated ultrasound neuromodulation for selectively shaping behavior in mice
    Article Snippet: .. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response. ary April 2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies: Chicken anti-MAP2 (PA1-10005, Invitrogen); Rabbit anti-Caspase-3 (#9661, Cell Signaling Technology); Rabbit anti-Iba-1 (#17198, Cell Signaling Technology); Rabbit anti-GFAP (#12389, Cell Signaling Technology); Rabbit anti-TPH2 (#51124, Cell Signaling Technology); Rabbit anti-c-Fos (#2250, Cell Signaling Technology). .. Secondary antibodies: Goat anti-chicken, Alexa Fluor 555 (A-21103, Invitrogen); Goat anti-rabbit, Alexa Fluor 488 (A-21428, Invitrogen).

    Incubation:

    Article Title: Orexin receptors 1 and 2 in serotonergic neurons differentially regulate peripheral glucose metabolism in obesity
    Article Snippet: .. Afterwards, they were incubated with primary antibodies, including goat anti-orexin A (1:250, sc-8070, goat pAb, Santa Cruz Biotechnology), chicken anti-GFP (1:1000, ab13970, chicken pAb, Abcam), rabbit anti-TPH2 (1:1000, #51124, rabbit mAb, Cell Signaling Technology) and/or rabbit anti-serotonin (1:400, S5545, rabbit pAb, Sigma-Aldrich), for overnight at RT, washed in PBS for 3 × 10 min, and incubated with secondary antibodies (1:500), including Alexa Fluro 594 donkey anti-goat, FITC donkey anti-chicken, Alexa Fluro 488 donkey anti-rabbit, Alexa Fluro 647 donkey anti-rabbit (a11058, sa 1-7200, a21206 and a31573, Invitrogen), for 1 h at RT. .. After washing in PBS for 3 × 10 min, slices were mounted and covered with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories).



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    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, <t>anti-TPH2</t> antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.
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    Image Search Results


    a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, anti-TPH2 antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.

    Journal: bioRxiv

    Article Title: Serotonergic neurons in the dorsal raphe nucleus scale O₂ utilization in response to traumatic stress

    doi: 10.64898/2026.01.14.699586

    Figure Lengend Snippet: a. Strategy for labeling DRN 5-HT neurons projecting to the VTA and the vCPU. AAV11-DIO-EGFP was bilaterally injected into the vCPU of Sert -Cre mice, and AAV11-DIO-mCherry was injected into both sides of the VTA. b. Images of retrogradely labeled DRN 5-HT neurons. Violet, anti-TPH2 antibody; mCherry⁺ cells, Sert DRN→VTA neurons; EGFP⁺ cells, Sert DRN→vCPU neurons. c. Percentages of three types of DRN 5-HT neurons in the DRN ( n = 7 mice). d. HyPer7 recordings of type III neurons. Strategy for labeling type III neurons and representative confocal image showing that type III neurons mainly locate in the ventral part of the DRN. e. Attack-locked rHF traces of type III neurons in response to a 10-day CSDS. The black lines indicate the onset of attacks. f. Mean attack-locked rHF of type III neurons in SUS and RES mice during a 10-day CSDS paradigm ( n = 6/12 in SUS/RES mice, respectively). g. HyPer7 recordings of Sert DRN→VTA neurons. h. Attack-locked rHF traces of Sert DRN→VTA neurons following a 10-day CSDS. i. Mean attack-locked rHF of type I neurons in SUS and RES mice ( n = 5/8 in SUS/RES mice). j. HyPer7 recordings of Sert DRN→vCPU neurons. k. Attack-locked rHF traces of Sert DRN→vCPU neurons following a 10-day CSDS. l. Mean attack-locked rHF of type II neurons in SUS and RES mice ( n = 6/6 in SUS/RES mice). Scale bars, 100 μm; * p < 0.05 and *** p < 0.001. f, i, l, two-way RM ANOVA with Sidak’s multiple comparisons test. # p < 0.05, # # p < 0.01, and # # # p < 0.001 relative to the basal level (dashed line) by one-sample t -test ( f, i, l ). n.s., not significant. Data are presented as mean ± SEM.

    Article Snippet: Free-floating sections were washed in PBS three times, 5 min/wash, permeabilized with 0.3% Triton X-100 in PBS for 30 min, blocked in blocking solution containing 5% normal goat serum and 0.3% Triton X-100 for 2 h at room temperature, and then stained with a rabbit polyclonal anti-TPH2 primary antibody (1:300, NB100-74555; Novus Biologicals) overnight at 4°C.

    Techniques: Labeling, Injection